Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF5665 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Neuroglycan C/CSPG5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat Neuroglycan C/CSPG5 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Neuroglycan C is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat Neuroglycan C/CSPG5 by Western Blot. Western blot shows lysates of rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Neuroglycan C/CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5665) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroglycan C/CSPG5 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuroglycan C/CSPG5 in Mouse Brain. Neuroglycan C/CSPG5 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Goat Anti-Mouse/Rat Neuroglycan C/CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5665) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuroglycan C/CSPG5 in Mouse Embryo. Neuroglycan C/CSPG5 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse/Rat Neuroglycan C/ CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5665) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.