H00002747-M01

antibody from Abnova Corporation

Targeting: GLUD2 GLUDP1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002747-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002747-M01, RRID:AB_10962182

Product name

GLUD2 monoclonal antibody (M01), clone 3C2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GLUD2.

Antigen sequence

MTPGFRDKTFVVQGFGNVGLHSMRYLHRFGAKCIA
VGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKA
KPYEGSILEVDCDILIPAATEKQLTKSNAPRVKAK
IIAEGANGPTTPEADKIFLERNILVIPDLYLNAGG
VTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLLS
VQESLERKFGKHGGTIPIVPTAEFQDSISGASEKD
IVHSALAYTMERSARQIMHTAMKYNLGLDLRTAAY
VNAIEKVFKVYSEAGVTFT


Isotype

IgG

Antibody clone number

3C2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol