Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14462-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MELAKKGFI GPGVDVPAPD MNTGEREMSW IADTYASTIG HYDINAHACV TGKPISQGGI HGRISATGRG VFHGIENFIN EASYMSILGM TPGFRDKTFV VQGFGNVGLH SMRYLHRFGA KCIAVGESDG SIWNPDGIDP KELEDFKLQH GSILGFPKAK PYEGSILEVD CDILIPAATE KQLTKSNAPR VKAKIIAEGA NGPTTPEADK IFLERNILVI PDLYLNAGGV TVSYFEWLKN LNHVSYGRLT FKYERDSNYH LLLSVQESLE RKFGKHGGTI PIVPTAEFQD SISGASEKDI VHSALAYTME RSARQIMHTA MKYNLGLDLR TAAYVNAIEK VFKVYSEAGV TFT (207-558 aa encoded by BC050732)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using GLUD2 antibody 14462-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GLUD2 (IP:14462-1-AP, 4ug; Detection:14462-1-AP 1:1000) with mouse liver tissue lysate 4000ug.