14462-1-AP

antibody from Invitrogen Antibodies

Targeting: GLUD2 GLUDP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14462-1-AP

Provider

Invitrogen Antibodies

Product name

GLUD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MELAKKGFI GPGVDVPAPD MNTGEREMSW IADTYASTIG HYDINAHACV TGKPISQGGI HGRISATGRG VFHGIENFIN EASYMSILGM TPGFRDKTFV VQGFGNVGLH SMRYLHRFGA KCIAVGESDG SIWNPDGIDP KELEDFKLQH GSILGFPKAK PYEGSILEVD CDILIPAATE KQLTKSNAPR VKAKIIAEGA NGPTTPEADK IFLERNILVI PDLYLNAGGV TVSYFEWLKN LNHVSYGRLT FKYERDSNYH LLLSVQESLE RKFGKHGGTI PIVPTAEFQD SISGASEKDI VHSALAYTME RSARQIMHTA MKYNLGLDLR TAAYVNAIEK VFKVYSEAGV TFT (207-558 aa encoded by BC050732)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GLUD2 antibody 14462-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GLUD2 (IP:14462-1-AP, 4ug; Detection:14462-1-AP 1:1000) with mouse liver tissue lysate 4000ug.