Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-19267 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GluD1/GluD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Functional evaluation of 16 SCHAD missense variants: Only amino acid substitutions causing congenital hyperinsulinism of infancy lead to loss-of-function phenotypes in vitro.
Velasco K, St-Louis JL, Hovland HN, Thompson N, Ottesen Å, Choi MH, Pedersen L, Njølstad PR, Arnesen T, Fjeld K, Aukrust I, Myklebust LM, Molven A
Journal of inherited metabolic disease 2021 Jan;44(1):240-252
Journal of inherited metabolic disease 2021 Jan;44(1):240-252
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamate Dehydrogenase in Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Samples were probed with the Glutamate Dehydrogenase antibody (Product # PA5-19267, 0.01µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamate Dehydrogenase by a Glutamate Dehydrogenase monoclonal antibody (Product # PA5-19267) at a concentration of 0.01 µg/mL. Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamate Dehydrogenase by a Glutamate Dehydrogenase monoclonal antibody (Product # PA5-19267) at a concentration of 0.03 µg/mL. Human (A) and Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Mouse Liver lysate using Product # PA5-19267 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Glutamate Dehydrogenase in paraffin embedded Human Cerebral Cortex tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the Glutamate Dehydrogenase antibody (Product # Pa5-19267) (3.8µg/mL) and AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GluD1 GluD2 in paraffin embedded human cerebral cortex using a GluD1 GluD2 polyclonal antibody (Product #PA5-19267) at a concentration of 3.75 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.