Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2957 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2957, RRID:AB_1570783
- Product name
- AARS polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AARS.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.
Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.
Ripmaster TL, Shiba K, Schimmel P
Proceedings of the National Academy of Sciences of the United States of America 1995 May 23;92(11):4932-6
Proceedings of the National Academy of Sciences of the United States of America 1995 May 23;92(11):4932-6
Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition.
Shiba K, Ripmaster T, Suzuki N, Nichols R, Plotz P, Noda T, Schimmel P
Biochemistry 1995 Aug 22;34(33):10340-9
Biochemistry 1995 Aug 22;34(33):10340-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AARS polyclonal antibody (Cat # PAB2957) in K-562 cell line lysates (35 ug/lane).AARS (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with AARS polyclonal antibody (Cat # PAB2957) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)