17394-1-AP

antibody from Invitrogen Antibodies

Targeting: AARS1 AARS, AlaRS, CMT2N

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17394-1-AP

Provider

Invitrogen Antibodies

Product name

AlaRS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDSTLTASE IRQRFIDFFK RNEHTYVHSS ATIPLDDPTL LFANAGMNQF KPIFLNTIDP SHPMAKLSRA ANTQKCIRAG GKHNDLDDVG KDVYHHTFFE MLGSWSFGDY FKELACKMAL ELLTQEFGIP IERLYVTYFG GDEAAGLEAD LECKQIWQNL GLDDTKILPG NMKDNFWEMG DTGPCGPCSE IHYDRIGGRD AAHLVNQDDP NVLEIWNLVF IQYNREADGI LKPLPKKSID TGMGLERLVS VLQNKMSNYD TDLFVPYFEA IQKGTGARPY TGKVGAEDAD GIDMAYRVLA DHARTITVAL ADGGRPDNTG RGYVLRRILR RAVRYAHEKL NASRGFFATL V (1-350 aa encoded by BC011451)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17394-1-AP (AlaRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AlaRS (IP:17394-1-AP, 4ug; Detection:17394-1-AP 1:500) with HepG2 cells lysate 2400ug.