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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29496 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alanyl-tRNA Synthetase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HepG2. Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (90%), Chicken (89%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Isolation of bacterial compartments to track movement of protein synthesis factors.
Zhao H, Martinis SA
Methods (San Diego, Calif.) 2017 Jan 15;113:120-126
Methods (San Diego, Calif.) 2017 Jan 15;113:120-126
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alanyl-tRNA Synthetase Polyclonal Antibody (Product # PA5-29496). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HepG2 . 7.5% SDS PAGE. alanyl-tRNA Synthetase Polyclonal Antibody (Product # PA5-29496) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alanyl-tRNA Synthetase Polyclonal Antibody (Product # PA5-29496). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HepG2 . 7.5% SDS PAGE. alanyl-tRNA Synthetase Polyclonal Antibody (Product # PA5-29496) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using alanyl-tRNA synthetase (Product # PA5-29496) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
