Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-111782 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GluR3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GluR3 was performed in rat cerebellum lysates. Lane 1: GluR3 (extracellular) Antibody (Product # PA5-111782) at a dilution of 1:400. Lane 2: GluR3 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GluR3 was performed in rat cerebellum lysates. Lane 1: GluR3 (extracellular) Antibody (Product # PA5-111782) at a dilution of 1:400. Lane 2: GluR3 (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GluR3 in frozen mouse cerebellum tissue sections using GluR3 (extracellular) Antibody (Product # PA5-111782). A) Distribution of GRIA3 (red). B) Distribution of glial fibrillary acidic protein (green). C) Merge of the two images indicates that GRIA3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GluR3 was performed in rat cerebellum lysates. 1) Cerebellum lysates. 2) Cerebellum lysates, GluR3 (extracellular) Antibody (Product # PA5-111782), and protein A beads. 3) Cerebellum lysates, pre-immune rabbit serum, protein A beads. Black arrow indicates GluR3 while the red arrow shows the IgG heavy chain. Immunoblot was performed with GluR3 (extracellular) Antibody.