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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- AF2886 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat HIF-2 alpha/EPAS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and rat HIF-2 alpha/EPAS1.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99814- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia.
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41.
Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc.
Martín-Aragón Baudel MAS, Rae MT, Darlison MG, Poole AV, Fraser JA
PloS one 2017;12(10):e0185664
PloS one 2017;12(10):e0185664
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081
Scientific reports 2017 Jul 11;7(1):5081
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41.
Bigot P, Colli LM, Machiela MJ, Jessop L, Myers TA, Carrouget J, Wagner S, Roberson D, Eymerit C, Henrion D, Chanock SJ
Nature communications 2016 Jul 7;7:12098
Nature communications 2016 Jul 7;7:12098
Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc.
Agrawal A, Gajghate S, Smith H, Anderson DG, Albert TJ, Shapiro IM, Risbud MV
Arthritis and rheumatism 2008 Dec;58(12):3798-808
Arthritis and rheumatism 2008 Dec;58(12):3798-808
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat HIF-2 alpha/EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2 for 16 h. PVDF membrane was probed with 1 µg/mL of Human/Rat HIF-2 alpha/EPAS1 Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2 alpha/EPAS1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HIF-2 alpha/EPAS1-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells treated with 150 μM CoCl2 overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-2 alpha/EPAS1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Rat HIF-2 alpha/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2886) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The oct-3/4 promoter was detected by standard PCR.
