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- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AF2997 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HIF-2 alpha/EPAS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat HIF-2 alpha/EPAS1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAH57870- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The lncRNA Neat1 promotes activation of inflammasomes in macrophages.
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.
Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin.
Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1α translation.
HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.
Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-β1 processing.
Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes.
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis.
Zhang P, Cao L, Zhou R, Yang X, Wu M
Nature communications 2019 Apr 2;10(1):1495
Nature communications 2019 Apr 2;10(1):1495
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.
Dao DT, Anez-Bustillos L, Jabbouri SS, Pan A, Kishikawa H, Mitchell PD, Fell GL, Baker MA, Watnick RS, Chen H, Rogers MS, Bielenberg DR, Puder M
PloS one 2018;13(12):e0208579
PloS one 2018;13(12):e0208579
Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin.
Kobayashi H, Liu Q, Binns TC, Urrutia AA, Davidoff O, Kapitsinou PP, Pfaff AS, Olauson H, Wernerson A, Fogo AB, Fong GH, Gross KW, Haase VH
The Journal of clinical investigation 2016 May 2;126(5):1926-38
The Journal of clinical investigation 2016 May 2;126(5):1926-38
Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
Milora KA, Fu H, Dubaz O, Jensen LE
The Journal of investigative dermatology 2015 Dec;135(12):2992-3000
The Journal of investigative dermatology 2015 Dec;135(12):2992-3000
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski DM
Journal of leukocyte biology 2014 Sep;96(3):365-75
Journal of leukocyte biology 2014 Sep;96(3):365-75
Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson TL, Rankin EB, Giaccia AJ, Schipani E
Developmental biology 2014 Sep 1;393(1):124-36
Developmental biology 2014 Sep 1;393(1):124-36
IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1α translation.
Dehne N, Tausendschön M, Essler S, Geis T, Schmid T, Brüne B
Journal of leukocyte biology 2014 Jan;95(1):129-37
Journal of leukocyte biology 2014 Jan;95(1):129-37
HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.
Weidemann A, Breyer J, Rehm M, Eckardt KU, Daniel C, Cicha I, Giehl K, Goppelt-Struebe M
Cell communication and signaling : CCS 2013 Oct 21;11:80
Cell communication and signaling : CCS 2013 Oct 21;11:80
Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-β1 processing.
Wottawa M, Leisering P, Ahlen Mv, Schnelle M, Vogel S, Malz C, Bordoli MR, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski DM
International journal of cancer 2013 Jun 15;132(12):2787-98
International journal of cancer 2013 Jun 15;132(12):2787-98
Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes.
Aro E, Khatri R, Gerard-O'Riley R, Mangiavini L, Myllyharju J, Schipani E
The Journal of biological chemistry 2012 Oct 26;287(44):37134-44
The Journal of biological chemistry 2012 Oct 26;287(44):37134-44
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC cancer 2012 May 29;12:201
BMC cancer 2012 May 29;12:201
Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis.
Stockmann C, Kerdiles Y, Nomaksteinsky M, Weidemann A, Takeda N, Doedens A, Torres-Collado AX, Iruela-Arispe L, Nizet V, Johnson RS
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 2;107(9):4329-34
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 2;107(9):4329-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat HIF-2 alpha/EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2 for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF-2 alpha/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2 alpha/EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-2 alpha/EPAS1 in Human Prostate Cancer Tissue. HIF-2 alpha/EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF-2 alpha/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-2 alpha/EPAS1 in Mouse Testis. HIF-2 alpha/EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF-2 alpha/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
