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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-119662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir3.4 (KCNJ5) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Kir3.4 on different lysates using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662) at 1:500 dilution. Positive control: Lane 1: Mouse heart ; Lane 2: Rat heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in 293T cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in HepG2 cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in LOVO cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat heart tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human lung cancer tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human pancreas tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse pancreas tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Kir3.4 (KCNJ5) in HepG2 cells using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662) at 1:100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
