H00004143-M01

antibody from Abnova Corporation

Targeting: MAT1A MAT, MATA1, SAMS, SAMS1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004143-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004143-M01, RRID:AB_1204669

Product name

MAT1A monoclonal antibody (M01), clone 4D11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant MAT1A.

Antigen sequence

MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICD
QISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE
ITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCN
VLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFG
YATDETEECMPLTIILAHKLNARMADLRRSGLLPW
LRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHN
EDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQ
PSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGG
AFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVL
VQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVH
KNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEF
PWEVPRKLVF


Isotype

IgG

Antibody clone number

4D11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAT1A expression in transfected 293T cell line by MAT1A monoclonal antibody (M01), clone 4D11.Lane 1: MAT1A transfected lysate(43.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAT1A is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of MAT1A transfected lysate using anti-MAT1A monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAT1A monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol