12970-1-AP

antibody from Invitrogen Antibodies

Targeting: OPRL1 KOR-3, NOCIR, NOPr, OOR, ORL1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12970-1-AP

Provider

Invitrogen Antibodies

Product name

OPRL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LVMYVILRH TKMKTATNIY IFNLALADTL VLLTLPFQGT DILLGFWPFG NALCKTVIAI DYYNMFTSTF TLTAMSVDRY VAICHPIRAL DVRTSSKAQA VNVAIWALAS VVGVPVAIMG SAQVEDEEIE CLVEIPTPQD YWGPVFAICI FLFSFIVPVL VISVCYSLMI RRLRGVRLLS GSREKDRNLR RITRLVLVVV AVFVGCWTPV QVFVLAQGLG VQPSSETAVA ILRFCTALGY VNSCLNPILY AFLDENFKAC FRKFCCASAL RRDVQVSDRV RSIAKDVALA CKTSETVPRP A (71-370 aa encoded by BC038433)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were stained with 0.2ug OPRL1 antibody (12970-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-OPRL1 (IP: 12970-1-AP, 4 µg; Detection: 12970-1-AP 1:500) with SH-SY5Y cells lysate 2000 µg.