Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- 720294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCHR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were transiently transfected with Melanin-concentrating hormone receptor 1/MCHR1 or Glucagon receptor/GCGR. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-Melanin concentrating hormone receptor 1/MCHR1 RAbbit Polyclonal Antibody (Product # 720294 at a concentration of 2 µg/mL). A band of 70 kDa corresponding to Melanin-concentrating hormone receptor 1/MCHR1 was observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were grown on poly-L-lysine-coated coverslips overnight. Cells were then transiently transfected with Melanin concentrating hormone receptor 1/MCHR1. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-Melanin concentrating hormone receptor 1/MCHR1 RAbbit Polyclonal Antibody (Product # 720294 at a concentration of 2 µg/mL. Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells and no staining of adjacent non-transfected cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Melanin concentrating hormone receptor 1/MCHR1 RAbbit Polyclonal Antibody (Product # 720294 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining is completely neutralized by preincubation of the antibody with its immunizing peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Melanin concentrating hormone receptor 1/MCHR1 RAbbit Polyclonal Antibody (Product # 720294 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining is completely neutralized by preincubation of the antibody with its immunizing peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human kidney were dewaxed, microwaved in citric acid and incubated with Anti-Melanin concentrating hormone receptor 1 / MCHR1 RAbbit Polyclonal Antibody (Product # 720294 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining is completely neutralized by preincubation of the antibody with its immunizing peptide.