711649

antibody from Invitrogen Antibodies

Targeting: MCHR1 GPR24, MCH1R, SLC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711649

Provider

Invitrogen Antibodies

Product name

MCHR1 Recombinant Polyclonal Antibody (20HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Mouse, Rabbit.

Antibody clone number

20HCLC

Concentration

0.5 mg/mL

References

Submitted references

An N-terminal fusion allele to study melanin concentrating hormone receptor 1.

Jasso KR, Kamba TK, Zimmerman AD, Bansal R, Engle SE, Everett T, Wu CH, Kulaga H, Reed RR, Berbari NF, McIntyre JC
Genesis (New York, N.Y. : 2000) 2021 Aug;59(7-8):e23438

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Melanin-Concentrating Hormone Receptor 1 (MCHR1) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-MCHR1 Recombinant Rabbit Polyclonal Antibody (Product # 711649 at a concentration of 1 µg/mL). A broad band from 60-70 kDa corresponding to MCHR1 was observed.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Melanin-Concentrating Hormone Receptor 1 (MCHR1) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-MCHR1 Recombinant Rabbit Polyclonal Antibody (Product # 711649 at a concentration of 1 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells.