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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-56663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SART1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TMILTLKDKG VLQEEEDVLV NVNLVDKERA EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGER
- Concentration
- 0.6 mg/mL
Submitted references SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes.
Yildirim A, Mozaffari-Jovin S, Wallisch AK, Schäfer J, Ludwig SEJ, Urlaub H, Lührmann R, Wolfrum U
Nucleic acids research 2021 Jun 4;49(10):5845-5866
Nucleic acids research 2021 Jun 4;49(10):5845-5866
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SART1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a SART1 Polyclonal Antibody (Product # PA5-56663).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SART1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a SART1 Polyclonal Antibody (Product # PA5-56663).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SART1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SART1 Polyclonal Antibody (Product # PA5-56663).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SART1 in human placenta shows strong nuclear positivity in trophoblastic cells. Samples were probed using a SART1 Polyclonal Antibody (Product # PA5-56663).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Effects of SANS depletion on Cajal bodies in HEK293T cells. (A-C) Double immunofluorescence staining for coilin (red) and PRPF31 in siCtrl- and siSANS- or siPRPF6-treated cells ( A ) and U5-52K ( B ) SART1 ( C ) in siCtrl- and siSANS-treated cells (for hSNU114 staining see Supplementary Figure S4D ). ( D ) Quantification of mean intensity ratios of PRPF31, U5-52K and SART1 signals observed in Cajal bodies reveals the accumulation of PRPF31 and SART1 but not U5-52K in Cajal bodies in SANS-depleted cells. (E-G) Analysis of RNA-FISH labelling of U4 ( E ), U5 ( F ), and U6 ( G ), snRNAs (green) in Cajal bodies (anti-coilin, red) in siCtrl- and siSANS-treated cells. ( H ) Quantification of the mean intensity ratios of accumulation of snRNAs in Cajal bodies. SANS depletion leads to the significant accumulation of U4 and U6 snRNAs but not U5 snRNA in CBs. Blue, DAPI counterstaining for nuclear DNA. *** P values < 0.001, ** P values < 0.01, * P values < 0.05.
