Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-3369-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD336 (NKp44) Monoclonal Antibody (44.189), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation. The 44.189 antibody has been reported to facilitate CD336 crosslinking. Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 44.189 antibody has been pre-titrated and tested by flow cytometric analysis of IL-2-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 44.189
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Natural cytotoxicity receptors: broader expression patterns and functions in innate and adaptive immune cells.
Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44.
Tumor-induced apoptosis of human IL-2-activated NK cells: role of natural cytotoxicity receptors.
NKp44 triggers NK cell activation through DAP12 association that is not influenced by a putative cytoplasmic inhibitory sequence.
NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily.
NKp44, a novel triggering surface molecule specifically expressed by activated natural killer cells, is involved in non-major histocompatibility complex-restricted tumor cell lysis.
Hudspeth K, Silva-Santos B, Mavilio D
Frontiers in immunology 2013;4:69
Frontiers in immunology 2013;4:69
Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44.
Rosental B, Brusilovsky M, Hadad U, Oz D, Appel MY, Afergan F, Yossef R, Rosenberg LA, Aharoni A, Cerwenka A, Campbell KS, Braiman A, Porgador A
Journal of immunology (Baltimore, Md. : 1950) 2011 Dec 1;187(11):5693-702
Journal of immunology (Baltimore, Md. : 1950) 2011 Dec 1;187(11):5693-702
Tumor-induced apoptosis of human IL-2-activated NK cells: role of natural cytotoxicity receptors.
Poggi A, Massaro AM, Negrini S, Contini P, Zocchi MR
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):2653-60
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):2653-60
NKp44 triggers NK cell activation through DAP12 association that is not influenced by a putative cytoplasmic inhibitory sequence.
Campbell KS, Yusa S, Kikuchi-Maki A, Catina TL
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):899-906
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 15;172(2):899-906
NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily.
Cantoni C, Bottino C, Vitale M, Pessino A, Augugliaro R, Malaspina A, Parolini S, Moretta L, Moretta A, Biassoni R
The Journal of experimental medicine 1999 Mar 1;189(5):787-96
The Journal of experimental medicine 1999 Mar 1;189(5):787-96
NKp44, a novel triggering surface molecule specifically expressed by activated natural killer cells, is involved in non-major histocompatibility complex-restricted tumor cell lysis.
Vitale M, Bottino C, Sivori S, Sanseverino L, Castriconi R, Marcenaro E, Augugliaro R, Moretta L, Moretta A
The Journal of experimental medicine 1998 Jun 15;187(12):2065-72
The Journal of experimental medicine 1998 Jun 15;187(12):2065-72
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells cultured in Human IL-2 Recombinant Protein Carrier-Free (Product # 34-8029-85) for 15 days with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD336 (NKp44) APC (right). Total viable cells were used for analysis.