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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- 10919-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SF3B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: DLYYEGKEF ETRLKEKKPG DLSDELRISL GMPVGPNAHK VPPPWLIAMQ RYGPPPSYPN LKIPGLNSPI PESCSFGYHA GGWGKPPVDE TGKPLYGDVF GTNAAEFQTK TEEEEIDRTP WGELEPSDEE SSEEEEEEES DEDKPDETGF ITPADSGLIT PGGFSSVPAG METPELIELR KKKIEEAMDG SETPQLFTVL PEKRTATVGG AMMGSTHIYD MSTVMSRKGP APELQGVEVA LAPEELELDP MAMTQKYEEH VREQQAQVEK EDFSDMVAEH AAKQKQKKRK AQPQDSRGGS KKYKEFKF (330-636 aa encoded by BC007610)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SF3B2 antibody (Product # 10919-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEK-293 cells using 10919-1-AP (SF3B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10919-1-AP (SF3B2 antibody) at dilution of 1:10 (under 40x lens).
