Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- NB100-79848 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79848, RRID:AB_1110399
- Product name
- Rabbit Polyclonal SF3B2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin.
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145.
Murthy T, Bluemn T, Gupta AK, Reimer M Jr, Rao S, Pillai MM, Minella AC
The Journal of biological chemistry 2018 Jun 29;293(26):10220-10234
The Journal of biological chemistry 2018 Jun 29;293(26):10220-10234
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
Zheng YZ, Xue MZ, Shen HJ, Li XG, Ma D, Gong Y, Liu YR, Qiao F, Xie HY, Lian B, Sun WL, Zhao HY, Yao L, Zuo WJ, Li DQ, Wang P, Hu X, Shao ZM
Cancer research 2018 Jun 15;78(12):3190-3206
Cancer research 2018 Jun 15;78(12):3190-3206
PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145.
Yang Y, Hadjikyriacou A, Xia Z, Gayatri S, Kim D, Zurita-Lopez C, Kelly R, Guo A, Li W, Clarke SG, Bedford MT
Nature communications 2015 Mar 4;6:6428
Nature communications 2015 Mar 4;6:6428
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SF3B2 Antibody [NB100-79848] - Detection of Human SF3b145/SAP145 from HeLa whole cell lysate (5, 15, 50 microgram) and 293T (50 microgram) using NB100-79848. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody NB100-79847.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SF3B2 Antibody [NB100-79848] - Section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). Detection: DAB