H00115727-M01

antibody from Abnova Corporation

Targeting: RASGRP4

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00115727-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00115727-M01, RRID:AB_606918

Product name

RASGRP4 monoclonal antibody (M01), clone 1F3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RASGRP4.

Antigen sequence

CGLCCHKHCRDQVKVECKKRPGAKGDAGPPGAPVP
STPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQ
TESPHPSWETDTVPCPVMDPPSTASSKLDS


Isotype

IgG

Antibody clone number

1F3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Galectin-3 regulates RasGRP4-mediated activation of N-Ras and H-Ras.

Shalom-Feuerstein R, Levy R, Makovski V, Raz A, Kloog Y
Biochimica et biophysica acta 2008 Jun;1783(6):985-93

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RASGRP4 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol