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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB12266 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NT5E monoclonal antibody, clone 4G6E3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human NT5E.
- Isotype
- IgG
- Antibody clone number
- 4G6E3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human NT5E (AA: 30-250) recombinant protein (Expected MW is 26.6 kDa) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NT5E (AA: 30-250)-hIgGFc transfected HEK293 cells] with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of A431 cell lysate with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of A431 cells with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
