4907-MSM1-P0

antibody from Invitrogen Antibodies

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

4907-MSM1-P0

Provider

Invitrogen Antibodies

Product name

CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E/2503)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

NT5E/2503

Vial size

20 µg

Concentration

200 µg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of A431 and PC-3 cell lysate using CD73 Mouse Monoclonal Antibody (NT5E/2503).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD73 Mouse Monoclonal Antibody (NT5E/2503) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD�s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SD�s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE analysis Purified CD73 Mouse Monoclonal Antibody (NT5E/2503). Confirmation of Purity and Integrity of Antibody.