Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00023063-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00023063-M02, RRID:AB_606480
- Product name
- KIAA0261 monoclonal antibody (M02), clone 2B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KIAA0261.
- Antigen sequence
MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKI
KEKIRRLCETVHNKHLDLENITTGHLAMETLLSLT
SKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRD
EDEEKLVASLWGAERCLRVLESVTVHNPENQSYLI
AYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLA
DSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTND
NEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRF
DIRVLLFLERERAAQLAESKTDELIKDAPTTQHDK
SGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEEL
DLNKALQHAGKHMEDCIVASYTALLLGCLCQESPI
NVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVG
TTGQKSISRVIEYLEHC- Isotype
- IgG
- Antibody clone number
- 2B3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KIAA0261 expression in transfected 293T cell line by KIAA0261 monoclonal antibody (M02), clone 2B3.Lane 1: KIAA0261 transfected lysate(45.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KIAA0261 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of WAPAL transfected lysate using anti-WAPAL monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with WAPAL monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol