H00002791-M01

antibody from Abnova Corporation

Targeting: GNG11 GNGT11

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002791-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002791-M01, RRID:AB_1137237

Product name

GNG11 monoclonal antibody (M01), clone 2H5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant GNG11.

Antigen sequence

MPALHIEDLPEKEKLKMEVEQLRKEVKLQRQQVSK
CSEEIKNYIEERSGEDPLVKGIPEDKNPFKEKGS


Isotype

IgG

Antibody clone number

2H5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GNG11 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between SMURF1 and GNG11. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-GNG11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)