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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF1579 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ATRIP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects endogenous human ATRIP in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q8WXE1- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils.
Nakagawa T, Natsume A, Satoh M, Niwa R
Leukemia research 2010 May;34(5):666-71
Leukemia research 2010 May;34(5):666-71
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse ATRIP by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATRIP at approximately 100 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ATRIP by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATRIP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
