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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6464 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6464, RRID:AB_1574837
- Product name
- FBXW2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of FBXW2.
- Antigen sequence
C-KRGSSFLAGEHPG- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A family of mammalian F-box proteins.
Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW
Current biology : CB 1999 Oct 21;9(20):1180-2
Current biology : CB 1999 Oct 21;9(20):1180-2
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FBXW2 polyclonal antibody (Cat # PAB6464) staining (0.3 ug/mL) of human kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FBXW2 polyclonal antibody (Cat # PAB6464) staining (3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
