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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-22035 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFA12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HepG2, Raji, mouse liver. Predicted reactivity: Mouse (88%), Rat (90%), Sheep (89%), Rhesus Monkey (94%), Chimpanzee (100%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.89 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFA12 Polyclonal Antibody (Product # PA5-22035). Sample (30 µg of whole cell lysate). A: Hep G2. 12% SDS PAGE. NDUFA12 Polyclonal Antibody (Product # PA5-22035) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFA12 Polyclonal Antibody (Product # PA5-22035). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 15% SDS PAGE. NDUFA12 Polyclonal Antibody (Product # PA5-22035) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DAP13 in methanol-fixed HepG2 cells using a DAP13 polyclonal antibody (Product # PA5-22035) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 (Product # PA5-22035) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NDUFA12 was performed in paraffin-embedded rat colon tissue using NDUFA12 Polyclonal Antibody (Product # PA5-22035) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12 (Product # PA5-22035) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
