25244-1-AP

antibody from Invitrogen Antibodies

Targeting: PHLPP2 KIAA0931, PHLPPL, PPM3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

25244-1-AP

Provider

Invitrogen Antibodies

Product name

PHLPPL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LDLQHNALT RLPDTLFSKA LNLRYLNASA NSLESLPSAC TGEESLSMLQ LLYLTNNLLT DQCIPVLVGH LHLRILHLAN NQLQTFPASK LNKLEQLEEL NLSGNKLKTI PTTIANCKRL HTLVAHSNNI SIFPEILQLP QIQFVDLSCN DLTEILIPEA LPATLQDLDL TGNTNLVLEH KTLDIFSHIT TLKIDQKPLP TTDSTVTS (575-781 aa encoded by BC129927)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25244-1-AP ( PHLPPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25244-1-AP ( PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PHLPPL (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:600) with NIH/3T3 cells lysate 2000ug.