NB100-1812

antibody from Novus Biologicals

Targeting: PHLPP2 KIAA0931, PHLPPL, PPM3B

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-1812

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1812, RRID:AB_2299549

Product name

Rabbit Polyclonal PHLPP2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Light activation of the insulin receptor regulates mitochondrial hexokinase. A possible mechanism of retinal neuroprotection.

Rajala A, Gupta VK, Anderson RE, Rajala RV
Mitochondrion 2013 Nov;13(6):566-76

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Biomarkers of response to Akt inhibitor MK-2206 in breast cancer.

Sangai T, Akcakanat A, Chen H, Tarco E, Wu Y, Do KA, Miller TW, Arteaga CL, Mills GB, Gonzalez-Angulo AM, Meric-Bernstam F
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Oct 15;18(20):5816-28

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PHLPP2 Antibody [NB100-1812] - Detection of Human and Mouse PHLPPL on HeLa whole cell lysate using NB100-1812. PHLPPL was also IPed, albeit less efficiently, using another rabbit anti-PHLPPL antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PHLPP2 Antibody [NB100-1812] - Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PHLPPL antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.