Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009477-B01P - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009477-B01P, RRID:AB_2142160
- Product name
- Mouse Polyclonal MED20 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. MED20 - mediator complex subunit 20,
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.
Sato S, Tomomori-Sato C, Tsai KL, Yu X, Sardiu M, Saraf A, Washburn MP, Florens L, Asturias FJ, Conaway RC, Conaway JW
The Journal of biological chemistry 2016 Dec 23;291(52):26886-26898
The Journal of biological chemistry 2016 Dec 23;291(52):26886-26898
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of MED20 expression in transfected 293T cell line by MED20 polyclonal antibody. Lane 1: TRFP transfected lysate(23.32 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of TRFP expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MED20 Antibody [H00009477-B01P] - Analysis of purified antibody to TRFP on HeLa cell. (antibody concentration 10 ug/ml)