Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAAB17140 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- EphB2 antibody - C - terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-EphB2 C-term Pab (Cat. # OAAB17140) in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EphB2 Antibody (C-term) (Cat# OAAB17140) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red) .DAPI was used to stain the cell nuclear (blue) .
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term) (Cat.# OAAB17140) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.