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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81348 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81348, RRID:AB_11162453
- Product name
- EphB2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of EphB2(arrow) using rabbit polyclonal EphB2 Antibody(GTX81348).293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the EphB2 gene (Lane 2) (Origene Technologies).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EphB2 antibody (GTX81348), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using EphB2 Antibody (bottom histogram)(GTX81348) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
