EB07253

antibody from Everest Biotech

Targeting: WNT3 INT4, MGC131950, MGC138321, MGC138323

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

EB07253

Provider

Everest Biotech

Proper citation

Everest Biotech Cat#EB07253, RRID:AB_2215286

Product name

Goat anti-WNT3 Antibody

Antibody type

Polyclonal

Description

Goat anti-WNT3 Antibody

Reactivity

Human

Host

Goat

Antigen sequence

CGRGHNTRTEKRKEK


Epitope

internal region

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07253 (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07253 (3.8µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.