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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-18516 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.
Wilkerson BA, Zebroski HL, Finkbeiner CR, Chitsazan AD, Beach KE, Sen N, Zhang RC, Bermingham-McDonogh O
eLife 2021 May 18;10
eLife 2021 May 18;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Brain lysate using Product # PA5-18516 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Breast using Product # PA5-18516 at a concentration of 3.8 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemichal analysis of WNT3 in Human Breast using a WNT3 polyclonal antibody (Product # PA5-18516) at a concentration of 2 µg/mL. The sample was paraffin embedded, and heat treated antigen retrieval was used to detect the target with HRP staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Cellular diversity in the nonsensory ampulla epithelium. ( a ) Highlights the position of the nonsensory epithelial cells of the ampulla in UMAP space relative to the whole dataset. ( b ) Cluster analysis in nonsensory ampulla epithelium. ( c ) Expression of markers relative to cell cluster and developmental time. Additional color bars indicate whether markers are known or novel in specificity or implicated in vestibular diseases and dysfunction. ( d ) The relative contributions of cell clusters to the nonsensory ampulla epithelium at the indicated developmental ages. ( e ) Wnt3 RNA ISH localizes to a distinct population of cells in top of the roof of the P1 Swiss Webster ampulla. ( f ) Wnt3 IF in vibratome-sectioned crista localizes to the top of the roof of the P0 B6 ampulla and extends into the semicircular canal. ( g ) Wnt3 IF in a whole mount P7 B6 crista bisected to show the apical surface of the rooftop en face. ( h ) Phalloidin staining in P3 B6 ampulla whole mount. Note that roof cells in the Wnt3 + domain are smaller than surrounding epithelial cells. Brackets in f-g indicate the Wnt3 + rooftop domain. Scale bars = 50 mum. Figure 4--figure supplement 1. Localization of cluster-specific markers in nonsensory ampulla epithelium. RNA in situ hybridization data from the Allen Developing Mouse Brain Atlas is shown for cluster-specific markers in E15.5 cristae. Insets show the cluster specificity in UMAP. Arrowheads indicate expression in the ampulla epithelium.
