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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-77311 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77311, RRID:AB_11021818
- Product name
- Rabbit Polyclonal Endosialin/CD248/TEM1 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77311 can detect human isoform 1, mouse and rat TEM-1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human Tissue Loading: 15 ug of lysates per lane. Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 ug of lysates per lane. Antibodies: TEM-1 NBP1-77310, (2 ug/mL), TEM-1 NBP1-77311, ( 1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue with TEM1 antibody at 2.5 ug/ml.
