16388-1-AP

antibody from Invitrogen Antibodies

Targeting: RIN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16388-1-AP

Provider

Invitrogen Antibodies

Product name

RIN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KSLHCSVLK PLRPILAARL RRRLAADGSL GRLAEGLRLA RAQGPGAFGS HLSLPSPVEL EQVRQKLLQL LRTYSPSAQV KRLLQACKLL YMALRTQEGE GAGADEFLPL LSLVLAHCDL PELLLEAEYM SELLEPSLLT GEGGYYLTSL SASLALLSGL GQAHTLPLSP VQELRRSLSL WEQRRLPATH CFQHLLRVAY QDPSSGCTSK TLAVPPEASI ATLNQLCATK FRVTQPNTFG LFLYKEQGYH RLPPGALAHR LPTTGYLVYR RAEWPETQGA VTEEEGSGQS EARSRGEEQG CQGDGDAGVK ASPRDIREQS ETTAEGGQGQ AQEGPAQPGE PEAEGSRAAE E (434-783 aa encoded by BC014417)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16388-1-AP (RIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16388-1-AP (RIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16388-1-AP (RIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16388-1-AP (RIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RIN1 (IP:16388-1-AP, 4ug; Detection:16388-1-AP 1:500) with HeLa cells lysate 3400ug.