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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP15154PU-N - Provider product page
- Provider
- OriGene
- Product name
- PFKFB4 rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- PFKFB4 rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PFKFB4
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FTS down-regulates HIF-1 and HIF-1-regulated proteins in GBM cells. GBM cell lines were incubated under hypoxia (1% O2) for the indicated periods with FTS and then processed for the determination of PFKFB4 by Western Blot. Samples of total cell lysates were also immunoblotted with anti-Beta-Tubulin antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analsis using PFKFB4 Antibody to detect PFKFB4 in mouse brain tissue lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Left: Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4?Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Right: Formalin-fixed, paraffin-embedded human cancer tissue reacted with PFKFB4?Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
- Validation comment
- IHC
