Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP2-30954 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MYL4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MYL4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MYL4 Antibody [NBP2-30954] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HEL
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: MYL4 Antibody [NBP2-30954] - Simple Western lane view shows a specific band for MYL4 in 0.2 mg/ml of HEL (left) and h. Heart (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: MYL4 Antibody [NBP2-30954] - Electropherogram image of the corresponding Simple Western lane view. MYL4 antibody was used at 1:50 dilution on HEL and h. Heart lysate(s) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in subsets of myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human colon shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows no positivity in myocytes as expected.