PA1-32415
antibody from Invitrogen Antibodies
Targeting: SCARB2
CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA1-32415 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SR-BI/SR-BII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The PA1-32415 immunogen is: Synthetic peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII was expressed as two tandem copies in bacteria using the pET system. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SR-BI/SR-BII was performed by loading 80 µg of mouse liver tissue lysates. Proteins were transferred to a membrane and probed with a SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415). Lane 1: wild-type mice , Lane 2: SR-BI deficient mice.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Scavenger Receptor SR-BI + SR-BII in Hela cells using a Scavenger Receptor SR-BI + SR-BII polyclonal antibody (Product # PA1-32415) and detected with a Dylight 488 labeled secondary antibody (Green). Nuclei were visualized using Hoechst 33258 counterstain (Blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SR-BI/SR-BII in HeLa cells using SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415) (Green). Red : Tubulin. Blue: DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SR-BI/SR-BII in human liver tissue using SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415) at a dilution of 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PEX19 was performed in NIH-3T3 cells using PEX19 Monoclonal Antibody (GT554) (Product # MA5-17266) (Purple) at a dilution of 1:400. M1 is defined by unstained cells.