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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- AF1888 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LIMPII/SR-B2 Lumenal Loop Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse LIMPII/SR-B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross-reactivity with rhCD36 and rhSR-B1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O35114- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains.
Identification of a human SCARB2 region that is important for enterovirus 71 binding and infection.
Casado ME, Huerta L, Ortiz AI, Pérez-Crespo M, Gutiérrez-Adán A, Kraemer FB, Lasunción MÁ, Busto R, Martín-Hidalgo A
Journal of lipid research 2012 Dec;53(12):2586-97
Journal of lipid research 2012 Dec;53(12):2586-97
Identification of a human SCARB2 region that is important for enterovirus 71 binding and infection.
Yamayoshi S, Koike S
Journal of virology 2011 May;85(10):4937-46
Journal of virology 2011 May;85(10):4937-46
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
