H00003034-M04

antibody from Abnova Corporation

Targeting: HAL HIS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003034-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003034-M04, RRID:AB_566289

Product name

HAL monoclonal antibody (M04), clone 4F2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HAL.

Antigen sequence

LAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIK
DRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEH
IPESRPLSPTAFSLQFLHKKSTKIPESEDL


Isotype

IgG

Antibody clone number

4F2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Increased sensitivity of histidinemic mice to UVB radiation suggests a crucial role of endogenous urocanic acid in photoprotection.

Barresi C, Stremnitzer C, Mlitz V, Kezic S, Kammeyer A, Ghannadan M, Posa-Markaryan K, Selden C, Tschachler E, Eckhart L
The Journal of investigative dermatology 2011 Jan;131(1):188-94

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Histidase expression in human epidermal keratinocytes: regulation by differentiation status and all-trans retinoic acid.

Eckhart L, Schmidt M, Mildner M, Mlitz V, Abtin A, Ballaun C, Fischer H, Mrass P, Tschachler E
Journal of dermatological science 2008 Jun;50(3):209-15

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody (M04), clone 4F2.Lane 1: HAL transfected lysate(72.698 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of HAL transfected lysate using anti-HAL monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HAL MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol