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LearnPA5-26365
antibody from Invitrogen Antibodies
Targeting: TRERF1
BCAR2, dJ139D8.5, HSA277276, RAPA, TReP-132
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-26365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TREF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a TREF1 polyclonal antibody (Product # PA5-26365) in K562 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using a TREF1 polyclonal antibody (Product # PA5-26365). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a TREF1 polyclonal antibody (Product # PA5-26365) (1:25, 1 hr at 37ºC). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37ºC). Actin filaments have been labeled with dye-conjugated phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of K562 cells using a TREF1 polyclonal antibody (Product # PA5-26365) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
