MA5-45749

antibody from Invitrogen Antibodies

Targeting: NPAS4 bHLHe79, Le-PAS, NXF, PASD10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-45749

Provider

Invitrogen Antibodies

Product name

NPAS4 Monoclonal Antibody (S408-79), APC

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S408-79

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Blocking: 2% GE Healthcare Blocker for 1 hour at RT. Samples were incubated with NPAS4 monoclonal antibody (Product # MA5-45749) at 1:1,000 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with NPAS4 monoclonal antibody (Product # MA5-45749) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 antibody (D) Composite.