Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-32006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFNAR1 Recombinant Rabbit Monoclonal Antibody (SR45-08)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SR45-08
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references 5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING.
Tian J, Zhang D, Kurbatov V, Wang Q, Wang Y, Fang D, Wu L, Bosenberg M, Muzumdar MD, Khan S, Lu Q, Yan Q, Lu J
The EMBO journal 2021 Apr 1;40(7):e106065
The EMBO journal 2021 Apr 1;40(7):e106065
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFNAR1 in SiHa cell lysate using a IFNAR1 Monoclonal antibody (Product # MA5-32006) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IFNAR1 of paraffin-embedded Human tonsil tissue using a IFNAR1 Monoclonal antibody (Product #MA5-32006). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IFNAR1 of paraffin-embedded Human spleen tissue using a IFNAR1 Monoclonal antibody (Product #MA5-32006). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IFNAR1 of paraffin-embedded rat brain tissue using a IFNAR1 Monoclonal antibody (Product #MA5-32006). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IFNAR1 of paraffin-embedded Mouse brain tissue using a IFNAR1 Monoclonal antibody (Product #MA5-32006). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of IFNAR1 in Jurkat cells using a IFNAR1 Monoclonal Antibody (Product # MA5-32006) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Efficient 5-FU-induced tumor inhibition depends on IFN-sensing by bone-marrow-derived cells A Western blot for Ctrl and Ifnar1-KO MC38 cells, analyzed with Ifnar1 and GAPDH antibodies. B Ctrl or Ifnar1-KO MC38 cells were treated with PBS or recombinant Ifnbeta for 4 h. The RNA expression levels of indicated ISGs were analyzed using qRT-PCR. N = 3. C-E Mice were injected with control (Ctrl) or Ifnar1-KO MC38 cells, and treated with 5-FU or PBS. (C) Pictures of tumors and spleens from a representative experiment. (D) Tumor volumes were quantified at the indicated days after cancer cell injection. N = 5. (E) Tumor and spleen weights at the endpoint for (D), with each dot representing a mouse. Ctrl tumor data in (C-E) are the same as those in Fig 4B-D. F-I Schematics (F) of the experiment to test the function of Ifnar1 in bone-marrow (BM)-derived cells. WT C57BL/6 mice were transplanted with either Ifnar1 +/+ or Ifnar1 -/- BM cells. Recipient mice were allowed to recover followed by the injection of WT MC38 cells, before treatment with 5-FU or PBS. (G) Pictures of tumors and spleens from a representative experiment. Image panels were cropped from the same picture. (H) Tumor volumes were quantified at the indicated days after cancer cell injection. N = 4 to N = 5, as shown in (G). (I) Tumor and spleen weights at the endpoint for (H), with each dot representing a mouse. Data information: For all panels, error bars stand for SD, and center values represent mean. Two-tailed