Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB300-258 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-258, RRID:AB_10002607
- Product name
- Rabbit Polyclonal IFN-alpha/beta R1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for IFNAR1 protein phosphorylated at Ser535,539.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor.
Kumar KG, Krolewski JJ, Fuchs SY
The Journal of biological chemistry 2004 Nov 5;279(45):46614-20
The Journal of biological chemistry 2004 Nov 5;279(45):46614-20
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFNAR1 [p Ser535, p Ser539] Antibody [NB300-258] - Detection of Flag immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT and 3. IFNAR1 S535A and S539A mutants. Detection with NB 300-258 is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide used as antigen but not by the dephospho form of this peptide.
