Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 13391-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAYVPAPGY QPTYNPTLPY YQPIPGGLNV GMSVYIQGVA SEHMKRFFVN FVVGQDPGSD VAFHFNPRFD GWDKVVFNTL QGGKWGSEER KRSMPFKKGA AFELVFIVLA EHYKVVVNGN PFYEYGHRLP LQMVTHLQVD GDLQLQSINF IGGQPLRPQG PPMMPPYPGP GHCHQQLNSL PTMEGPPTFN PPVPYFGRLQ GGLTARRTII IKGYVPPTGK SFAINFKVGS SGDIALHINP RMGNGTVVRN SLLNGSWGSE EKKITHNPFG PGQFFDLSIR CGLDRFKVYA NGQHLFDFAH RLSAFQRVDT LEIQGDVTLS YVQI (1-323 aa encoded by BC034750)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 13391-1-AP (Galectin-4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug Galectin-4 antibody (13391-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.