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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 27552-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GWDKVVFNT LQGGKWGSEE RKRSMPFKKG AAFELVFIVL AEHYKVVVNG NPFYEYGHRL PLQMVTHLQV DGDLQLQSIN FIGGQPLRPQ GPPMMPPYPG PGHCHQQLNS LPTMEGPPTF NPPVPYFGRL QGGLTARRTI IIKGYVPPTG KSFAINFKVG SSGDIALHIN PR (70-240 aa encoded by BC034750)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- mouse colon tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Galectin-4 antibody (Product # 27552-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 27552-1-AP ( LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 27552-1-AP ( LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
