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- References [1]
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- NBP1-00219 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00219, RRID:AB_1503818
- Product name
- Goat Polyclonal NRXN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_004792.1 and NP_620072.1).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references High frequency of neurexin 1beta signal peptide structural variants in patients with autism.
Feng J, Schroer R, Yan J, Song W, Yang C, Bockholt A, Cook EH Jr, Skinner C, Schwartz CE, Sommer SS
Neuroscience letters 2006 Nov 27;409(1):10-3
Neuroscience letters 2006 Nov 27;409(1):10-3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with NBP1-00219 at 0.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with (1/3000) in lane C. Mock-transfected HEK293 probed with NBP1-00219 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NRXN1 Antibody [NBP1-00219] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
