14681-1-AP

antibody from Invitrogen Antibodies

Targeting: ASNS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14681-1-AP

Provider

Invitrogen Antibodies

Product name

ASNS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LMTDRRIGC LLSGGLDSSL VAATLLKQLK EAQVQYPLQT FAIGMEDSPD LLAARKVADH IGSEHYEVLF NSEEGIQALD EVIFSLETYD ITTVRASVGM YLISKYIRKN TDSVVIFSGE GSDELTQGYI YFHKAPSPEK AEEESERLLR ELYLFDVLRA DRTTAAHGLE LRVPFLDHRF SSYYLSLPPE MRIPKNGIEK HLLRETFEDS NLIPKEILWR PKEAFSDGIT SVKNSWFKIL QEYVEHQVDD AMMANAAQKF PFNTPKTKEG YYYRQVFERH YPGRADWLSH YWMPKWINAT DPSARTLTHY KSAVKA (247-561 aa encoded by BC014621)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14681-1-AP (ASNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14681-1-AP (ASNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of ASNS antibody (14681-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASNS transfected Jurkat cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 14681-1-AP (ASNS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ASNS (IP:14681-1-AP, 4ug; Detection:14681-1-AP 1:500) with HeLa cells lysate 1200ug.