Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX60466 - Provider product page
- Provider
- GeneTex
- Product name
- alpha Smooth Muscle Actin antibody [4A4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
Submitted references Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, Driver I, Rock J, Goga A, Khan SA, Lawson DA, Werb Z, Kessenbrock K
Nature communications 2018 May 23;9(1):2028
Nature communications 2018 May 23;9(1):2028
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Supportive validation
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- Experimental details
- WB analysis of (1) HeLa, (2) A431, (3) Jurkat, (4) K562, (5) HEK293, (6) HepG2, (7) NIH/3T3, (8) PC-12 and (9) Cos7 lysates using alpha Smooth Muscle Actin antibody [4A4].
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ELISA analysis of control antigen and antigen using alpha Smooth Muscle Actin antibody [4A4].
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HepG2 cells using alpha Smooth Muscle Actin antibody [4A4] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human duodenum tissues (left) and human esophagus tissues (right) using alpha Smooth Muscle Actin antibody [4A4].
Supportive validation
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- GeneTex (provider)
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- Experimental details
- FACS analysis of HeLa cells using alpha Smooth Muscle Actin antibody [4A4] (green) or a negative control (purple).